Aotelega | fa'aaoga e su'e ai le fa'ama'i o le Avian leukosis P27 i le toto manu, feces, cloaca, ma le fuamoa pa'epa'e. |
Mataupu Faavae | Avian Leukosis (AL) P27 antigen Elisa kit o lo'o fa'aaogaina e su'e ai le fa'ama'i leukosis Avian P27 i le toto manu, feces, cloaca, ma le fuamoa pa'epa'e.
|
Su'esu'ega Sini | Leukosis Avian (AL) P27 antigen |
Fa'ata'ita'iga | Serum
|
Tele | 1 pusa = 192 Su'ega |
Maumau ma Teuina | 1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃.Aua le aisa. 2) E 12 masina le ola fa'asa.Fa'aaogā uma mea fa'afofoga a'o le'i uma le aso i le pusa.
|
Avian Leukosis (AL) ose fa'aupuga tu'ufa'atasia mo fa'ama'i tumo eseese i moa e mafua mai i le Avian Leukosis Virus (ALV) i le aiga Retroviridae.O lenei fa'ama'i e fa'asalalauina i le lalolagi atoa ma e maualuga le fa'ama'i.E mafai ona mafua ai le oti ma le paʻu o moa, faʻaitiitia le gaosiga o le lafu, ma o se tasi o faʻamaʻi autu e matua lamatia ai le atinaʻeina o pisinisi moa.O lenei faʻamaʻi e umi se tala faʻasolopito ma o loʻo faʻaalia pea i taimi uma ni mataupu fou, e pei o le Avian leukemia virus subgroup J (ALV-J), lea na maua ma faʻaalia i le faaiuga o le 1980s i Peretania o se vaega fou o le avian leukemia virus, ma mafua ai le tele. afaina ai le pisinisi fai moa
O lo'o fa'aogaina e le pusa se sanuisi auala ELISA, fa'amama le anti-avian leukocyte P27 monoclonal antibody o lo'o fa'apipi'iina muamua i luga ole enzyme micro-well strips.I le su'ega, o le antigen i totonu o le fa'ata'ita'iga o lo'o fusifusia i le antibody i luga o le ipu ufiufi, pe a uma ona fufulu e aveese. o le antigen unbound ma isi vaega, o le enzyme monoclonal antibody ua faʻaopoopoina e faʻapipiʻi faapitoa i le faʻalavelave faʻamaʻi-antibody i luga o le ipu suʻega.ona fufulu lea, ua aveese le conjugate enzyme unbound, TMB substrate solution ua faaopoopo i le microplate, o le faailo lanumoana e le Enzyme catalysis o le saʻo saʻo o le anotusi antibody i le faʻataʻitaʻiga.Fa'aopoopo le vaifofo taofi, A mae'a le tali, o le absorbance O le tau o le tali lelei e fuaina i le galu o le 450 nm.
Reagent | Volume 96 Su'ega/192Su'ega | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Faatonuga | 1 pcs |