Oloa
Avian Leukemia P27 Antigen ELISA Kit
ELISA Kit Fa'ama'i Fa'ama'i Hydatid
| Aotelega | fa'aaoga e su'e ai le fa'ama'i o le Avian leukosis P27 i le toto manu, feces, cloaca, ma le fuamoa pa'epa'e. |
| Mataupu Faavae | Avian Leukosis (AL) P27 antigen Elisa kit o lo'o fa'aaogaina e su'e ai le fa'ama'i leukosis Avian P27 i le toto manu, feces, cloaca, ma le fuamoa pa'epa'e.
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| Su'esu'ega Sini | Leukosis Avian (AL) P27 antigen |
| Fa'ata'ita'iga | Serum
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| Tele | 1 pusa = 192 Su'ega |
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Maumau ma Teuina | 1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃. Aua le aisa. 2) E 12 masina le ola fa'asa. Fa'aoga uma mea fa'afofoga a'o le'i uma le aso i le pusa.
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Fa'amatalaga
Avian Leukosis (AL) ose fa'aupuga tu'ufa'atasia mo fa'ama'i tumo eseese i moa e mafua mai i le Avian Leukosis Virus (ALV) i le aiga Retroviridae. O lenei fa'ama'i e fa'asalalauina i le lalolagi atoa ma e maualuga le fa'ama'i. E mafai ona mafua ai le oti ma le paʻu o moa, faʻaitiitia le gaosiga o le lafu, ma o se tasi lea o faʻamaʻi autu e matua lamatia ai le atinaʻeina o pisinisi moa. O lenei faʻamaʻi e umi se talaʻaga ma o loʻo feagai pea ma faʻalavelave fou, e pei o le Avian leukemia virus subgroup J (ALV-J), lea na maua ma faʻaalia i le faaiuga o le 1980s i Peretania o se subtype fou o le avian leukemia virus, ma mafua ai le afaina tele i le pisinisi broiler.
Mataupu Faavae o le Su'ega
O le pusa o loʻo faʻaaogaina se metotia ELISA sanuisi, faʻamaʻi le anti-avian leukocyte P27 monoclonal antibody ua muaʻi faʻapipiʻiina i luga ole enzyme micro-well strips.I le suʻega, o le antigen i le faʻataʻitaʻiga o loʻo fusifusia i le antibody i luga o le ipu faʻapipiʻi, pe a uma ona fufulu e aveese le antigen unbound ma isi vaega, e faʻaopoopo le enzyme monoclonal antibody e faʻapipiʻi faʻapitoa i le antigen-test antibody. ona fufulu lea, ua aveese le conjugate enzyme unbound, TMB substrate solution ua faaopoopo i le microplate, o le faailo lanumoana e le Enzyme catalysis o le saʻo saʻo o le anotusi antibody i le faʻataʻitaʻiga. Fa'aopoopo le vaifofo taofi, A mae'a le tali, o le absorbance O le tau o le tali lelei e fuaina i le galu o le 450 nm.
Anotusi
| Reagent | Volume 96 Su'ega/192Su'ega | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Faatonuga | 1 pcs |
