Aotelega | Su'esu'eina ole Antibody fa'apitoa ole Fa'ama'i vae ma le gutu ole BRU |
Mataupu Faavae | O le BRU antibody ELISA pusa suʻega e faʻaaogaina e suʻesuʻe ai le Brucellosis antibodies i le toto o puaa, povi, mamoe ma 'oti. |
Su'esu'ega Sini | O le BRU antibody |
Fa'ata'ita'iga | Serum
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Tele | 1 pusa = 192 Su'ega |
Maumau ma Teuina | 1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃.Aua le aisa. 2) E 12 masina le ola fa'asa.Fa'aaogā uma mea fa'afofoga a'o le'i uma le aso i le pusa.
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Brucellosisis ose pepesi silizoonosismafua mai i le inuina o mea e le'i fa'asalainasusupe le velaaano o manumai manu fa'ama'i, po'o feso'ota'iga vavalalata ma a latou mea lilo.
E lauiloa foi o le fiva undulant, fiva Malta, ma le fiva Metitirani.
Ole BRU antibodyELISApusa su'egafaaaoga e suʻesuʻeina o faʻamaʻi Brucellosis i le serum o puaa, povi, smamoe ma 'oti .
Fa'aaoga lenei pusa faiga faatauva ELISA e mua'i ofuinaed BRU antigens i luga o vaieli microplate.A'o su'esu'e, fa'aopoopo i ai se fa'ata'ita'iga o le su'a fa'afefeteina maenzyme fa'ailoga anti-BRU monoclonal antibody, pe a uma ona incubation, pe a iai maua BRU antibody, o le a tuʻufaʻatasia ma le antigen muaʻi ufiufi, antibody i le faʻataʻitaʻiga poloka le tuʻufaʻatasia o le antibody monoclonal ma le antigen muaʻi ufiufi;tia'i le fa'atasi ole enzyme fa'atasi ma le fufulu;Fa'aopoopo le mea'ai TMB i totonu o vaieli laiti, o le faailo lanumoana e le Enzyme catalysis o lo'o i totonu o le fa'afeagai o le anotusi o le antibody i totonu o fa'ata'ita'iga..
Reagent | Volume 96 Su'ega/192Su'ega | ||
1 |
| 1ea/2ea | |
2 |
| 2ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Faatonuga | 1 pcs |