Oloa- fu'a

Oloa

Chicken Infectious Bursal Disease Virus Ab Elisa kit

Code o oloa:

Igoa Mea: Chicken Infectious Bursal Disease Virus Ab Elisa kit

Aotelega: Chicken infectious bursal disease virus antibody detection kit mo le su'esu'eina o moa infectious bursal disease virus neutralizing antibody i totonu o le moa moa mo le iloiloina o antibodies e fa'afitia e gaosia e moa fa'ama'i pipisi tui tui Tulaga ma fesoasoani serologically su'esu'ega o moa ua a'afia.

Sa'ili'iliga: Momoa fa'ama'i pipisi fa'ama'i fa'ama'i fa'ama'i fa'ama'i

Fa'ata'ita'iga Su'ega: Serum

Faʻamatalaga: 1 pusa = 192 Suʻega

Teuina: Reagents uma e tatau ona teuina i le 2 ~ 8 ℃.Aua le aisa.

Taimi Fa'ataga: 12 masina.Fa'aaogā uma mea fa'afofoga a'o le'i uma le aso i le pusa.


Fa'amatalaga Oloa

Faailoga o oloa

Chicken Infectious Bursal Disease Virus Ab Elisa kit

Aotelega  Su'esu'ega o le fa'a'ese'ese o le antibody e fa'asaga i le fa'ama'i pipisi ole fabricius virus ile sua moa
Su'esu'ega Sini Manoa fa'ama'i pipisi fa'ama'i virus antibody
Fa'ata'ita'iga Serum

 

Tele 1 pusa = 192 Su'ega
 

 

Maumau ma Teuina

1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃.Aua le aisa.

2) E 12 masina le ola fa'asa.Fa'aaogā uma mea fa'afofoga a'o le'i uma le aso i le pusa.

 

 

 

Fa'amatalaga

Fa'ama'i pipisi(IBD), e lauiloa foi o le faamaʻi Gumboro, bursitis faʻamaʻi ma faʻamaʻi avian nephrosis, o se faʻamaʻi pipisi tele o talavou.moama pipi e mafua mai i siama fa'ama'i pipisi (IBDV), fa'aalia eimmunosuppressionma le oti masani i le 3 i le 6 vaiaso le matua.O le faamai na maua muamua iGumboro, Delawarei le 1962. E taua tele le tamaoaiga i pisinisi moa i le lalolagi atoa ona o le faʻateleina o le faʻamaʻi i isi faʻamaʻi ma le faʻalavelave le lelei i le lelei.tui.I tausaga talu ai nei, ua aliaʻe mai i Europa ia faʻamaʻi leaga tele o le IBDV (vvIBDV), e mafua ai le oti tele o moa,Amerika Latina,Asia i Sautesasae, Aferika ma leSasa'e Tutotonu.O le fa'ama'i e ala i le ala oro-fecal, ma o manu ua a'afia e alu ese mai le maualuga o le siama mo le tusa ma le 2 vaiaso talu ona fa'ama'i.O le fa'ama'i e faigofie ona sosolo mai moa ua pisia i moa maloloina e ala i mea'ai, vai, ma fa'afeso'ota'i tino.

Mataupu Faavae o le Su'ega

O lo'o fa'aogaina e le pusa se faiga fa'atauva ELISA, fa'ama'i fa'ama'i fa'ama'i fa'ama'i VP2 polotini i luga o le microplate, ma fa'atauva ma le anti-VP2 protein antibody i totonu o le serum mo le fa'amalo o le fa'aogaina o le anti-VP2 protein monoclonal antibody.I le suʻega, o le monoclonal antibody e faʻataʻitaʻiina ma faʻaopoopoina se polotini anti-VP2, ma a maeʻa le faʻamaʻiina, afai o le faʻataʻitaʻiga o loʻo i ai le moa faʻamaʻi pipisi siama virus VP2 protein-specific antibody, e fusifusia i le antigen i luga o le ipu ufiufi.O le mea lea o le polokaina o le fusifusia o le anti-VP2 protein monoclonal antibody i le antigen, pe a uma ona fufulu e aveese le antibody le fusifusia ma isi vaega;ona fa'aopoopoina lea o le anti-mouse enzyme-label antibody lona lua e fa'apipi'i fa'apitoa i le antigen-antibody complex i luga o le mea e iloa ai;O le conjugate enzyme e le'i noatia e aveese e ala i le fufuluina;le TMB substrate ua faaopoopo i le microwell e atiina ae lanu, ma o le absorbance tau o le faataitaiga e le lelei faamaopoopo ma le anotusi o le anti-VP2 porotini antibody o loo i ai, ma ausia ai le faamoemoega o le sailia o le anti-VP2 protein antibody i le faataitaiga.

Mataupu

 

Reagent

Volume

96 Su'ega/192Su'ega

1
Microplate ua ufiufi antigen

 

1ea/2ea

2
 Pule Leaga

 

2.0ml

3
 Pulea lelei

 

1.6ml

4
 Fa'ata'ita'i vaiinu

 

100ml

5
Fufulu vaifofo (10X concentrate)

 

100ml

6
 Enzyme conjugate

 

11/22ml

7
 Alafua

 

11/22ml

8
 Taofi fofo

 

15ml

9
Fa'amau fa'amau fa'amau

 

2ea/4ea

10 serum dilution microplate

1ea/2ea

11  Faatonuga

1 pcs

 


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