Aotelega | Su'esu'ega o le fa'a'ese'ese o le antibody e fa'asaga i le fa'ama'i pipisi ole fabricius virus ile sua moa |
Su'esu'ega Sini | Manoa fa'ama'i pipisi fa'ama'i virus antibody |
Fa'ata'ita'iga | Serum
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Tele | 1 pusa = 192 Su'ega |
Maumau ma Teuina | 1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃.Aua le aisa. 2) E 12 masina le ola fa'asa.Fa'aaogā uma mea fa'afofoga a'o le'i uma le aso i le pusa.
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Fa'ama'i pipisi(IBD), e lauiloa foi o le faamaʻi Gumboro, bursitis faʻamaʻi ma faʻamaʻi avian nephrosis, o se faʻamaʻi pipisi tele o talavou.moama pipi e mafua mai i siama fa'ama'i pipisi (IBDV), fa'aalia eimmunosuppressionma le oti masani i le 3 i le 6 vaiaso le matua.O le faamai na maua muamua iGumboro, Delawarei le 1962. E taua tele le tamaoaiga i pisinisi moa i le lalolagi atoa ona o le faʻateleina o le faʻamaʻi i isi faʻamaʻi ma le faʻalavelave le lelei i le lelei.tui.I tausaga talu ai nei, ua aliaʻe mai i Europa ia faʻamaʻi leaga tele o le IBDV (vvIBDV), e mafua ai le oti tele o moa,Amerika Latina,Asia i Sautesasae, Aferika ma leSasa'e Tutotonu.O le fa'ama'i e ala i le ala oro-fecal, ma o manu ua a'afia e alu ese mai le maualuga o le siama mo le tusa ma le 2 vaiaso talu ona fa'ama'i.O le fa'ama'i e faigofie ona sosolo mai moa ua pisia i moa maloloina e ala i mea'ai, vai, ma fa'afeso'ota'i tino.
O lo'o fa'aogaina e le pusa se faiga fa'atauva ELISA, fa'ama'i fa'ama'i fa'ama'i fa'ama'i VP2 polotini i luga o le microplate, ma fa'atauva ma le anti-VP2 protein antibody i totonu o le serum mo le fa'amalo o le fa'aogaina o le anti-VP2 protein monoclonal antibody.I le suʻega, o le monoclonal antibody e faʻataʻitaʻiina ma faʻaopoopoina se polotini anti-VP2, ma a maeʻa le faʻamaʻiina, afai o le faʻataʻitaʻiga o loʻo i ai le moa faʻamaʻi pipisi siama virus VP2 protein-specific antibody, e fusifusia i le antigen i luga o le ipu ufiufi.O le mea lea o le polokaina o le fusifusia o le anti-VP2 protein monoclonal antibody i le antigen, pe a uma ona fufulu e aveese le antibody le fusifusia ma isi vaega;ona fa'aopoopoina lea o le anti-mouse enzyme-label antibody lona lua e fa'apipi'i fa'apitoa i le antigen-antibody complex i luga o le mea e iloa ai;O le conjugate enzyme e le'i noatia e aveese e ala i le fufuluina;le TMB substrate ua faaopoopo i le microwell e atiina ae lanu, ma o le absorbance tau o le faataitaiga e le lelei faamaopoopo ma le anotusi o le anti-VP2 porotini antibody o loo i ai, ma ausia ai le faamoemoega o le sailia o le anti-VP2 protein antibody i le faataitaiga.
Reagent | Volume 96 Su'ega/192Su'ega | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Faatonuga | 1 pcs |