Chicken Infectious Bursal Disease Virus Ab Elisa kit

O lo'o fa'aogaina e le pusa se faiga fa'atauva ELISA, fa'ama'i fa'ama'i fa'ama'i fa'ama'i VP2 polotini i luga o le microplate, ma fa'atauva ma le anti-VP2 protein antibody i totonu o le toto mo le fa'amalo fa'amalo e fa'aaoga ai le anti-VP2 protein monoclonal antibody.I le suʻega, o le monoclonal antibody e faʻataʻitaʻiina ma faʻaopoopoina se polotini anti-VP2, ma a maeʻa le faʻamaʻiina, afai o le faʻataʻitaʻiga o loʻo i ai le moa faʻamaʻi pipisi siama virus VP2 protein-specific antibody, e fusifusia i le antigen i luga o le ipu ufiufi.O le mea lea o le polokaina o le fusifusia o le anti-VP2 protein monoclonal antibody i le antigen, pe a uma ona fufulu e aveese le antibody le fusifusia ma isi vaega;ona fa'aopoopoina lea o le anti-mouse enzyme-labeled antibody lona lua e pipii fa'apitoa i le antigen-antibody complex i luga o le mea e iloa ai;O le enzyme conjugate e le'i noatia e aveese e ala i le fufuluina;ua faaopoopo le substrate TMB i le microwell e atiina ae lanu, ma o le absorbance taua o le faataitaiga e le lelei faamaopoopo ma le anotusi o le anti-VP2 protein antibody o loo i ai, ma ausia ai le faamoemoega o le sailia o le anti-VP2 protein antibody i le faataitaiga.