Aotelega | Su'esu'ega o le fa'ama'i fa'ama'i fa'apitoa o le Tuberculosis Bovine (BTB). |
Mataupu Faavae | Bovine Tuberculosis(BTB) antibody Elisa pusa su'ega e mafai ona fa'aoga e su'e ai le fa'ama'i fa'ama'i o le Tuberculosis Bovine i totonu ole toto po'o le plasma ole Bovine. |
Su'esu'ega Sini | Bovine Tuberculosis(BTB) antibody |
Fa'ata'ita'iga | Serum
|
Tele | 1 pusa = 192 Su'ega |
Maumau ma Teuina | 1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃.Aua le aisa. 2) E 12 masina le ola fa'asa.Fa'aaogā uma mea fa'afofoga a'o le'i uma le aso i le pusa.
|
O le Mycobacterium bovis ose siama e fa'atupu telegese (16- i le 20-itula taimi fa'atupu) fa'ama'i aerobic ma le fa'ama'i e mafua ai le mamapala i povi (ua ta'ua o le TB bovine).E feso'ota'i ma le Mycobacterium tuberculosis, le siama e mafua ai le mamapala i tagata.E mafai e le M. bovis ona oso i le pa puipui o le ituaiga ma mafua ai le fa'ama'i pei o le mamapala i tagata ma isi meaola.
Lenei pusa fa'aoga lē tuusao ELISA auala, mama BTB antigen is mua'i ufiufi on enzyme micro-vaieli fasipepa. Pe a suʻeina, faʻaopoopo fa'afefeteina sua fa'ata'ita'iga, mulimuli ane incubation, if iina is BTB patino antibody, it loto fa'atasi ma o le mua'i ufiufi antigen, lafoai o le lē tu'ufa'atasia antibody ma isi vaega ma fufulu; ona fa'aopoopo enzyme fa'atasi, lafoai o le lē tu'ufa'atasia enzyme fa'atasi ma
fufulu. Fa'aopoopo le TMB substrate i micro-wells, o le faailo lanumoana e le Enzyme catalysis e sa'o vaega ole antibody i totonu ole faataitaiga.
Reagent | Volume 96 Su'ega/192Su'ega | ||
1 |
| 1ea/2ea | |
2 |
| 2.0ml | |
3 |
| 1.6ml | |
4 |
| 100ml | |
5 |
| 100ml | |
6 |
| 11/22ml | |
7 |
| 11/22ml | |
8 |
| 15ml | |
9 |
| 2ea/4ea | |
10 | serum dilution microplate | 1ea/2ea | |
11 | Faatonuga | 1 pcs |