Oloa
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
Eggs Drop Syndrome1976 virus Antibody ELISA Ki
| Aotelega | Use e iloa ai le antibody faʻapitoa e faasaga i le EDS76 i serum qualitatively. |
| Mataupu Faavae | O le Eggs Drop Syndrome 1976 virus(EDS76) Ab Elisa kit e fa'aoga e su'e ai fa'ama'i fa'apitoa e fa'asaga i le EDS76 i le toto i se tulaga fa'apitoa.Mo le mataʻituina o le antibody pe a maeʻa le EDS76 puipuia ma faʻamaʻi faʻamaʻi o faʻamaʻi ile Avian. |
| Su'esu'ega Sini | Antibody e faasaga i le EDS76 i le toto |
| Fa'ata'ita'iga | Serum
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| Tele | 1 pusa = 192 Su'ega |
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Maumau ma Teuina | 1) E tatau ona teuina uma reagents i le 2 ~ 8 ℃.Aua le aisa. 2) E 12 masina le ola fa'asa.Fa'aoga uma mea fa'afofoga a'o le'i uma le aso i le pusa.
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Fa'amatalaga
Egg Drop Syndrome (EDS-76) ose fa'ama'i fa'ama'i pipisi e mafua mai i le adenovirus vaega III o le Adenoviridae Avian Virus genus ma le hemagglutination.I nisi o faatoaga moa, o le tele o fuamoa fuamoa o moa na faʻafuaseʻi ona faʻaitiitia, ma fuamoa faʻaleagaina e pei ole atigi vaivai, fuamoa e leai ni atigi, ma fuamoa manifinifi na gaosia ile taimi e tasi.O le faʻamaʻi atoa o le faʻamaʻi e tumau mo le 5-6 vaiaso, pe a maeʻa ona faʻasolosolo malie le fua o fuamoa, ae e faigata ona oʻo i le maualuga aʻo leʻi pa'ū.
Mataupu Faavae o le Su'ega
O lenei pusa o loʻo faʻaogaina le auala ELISA le tuʻusaʻo, faʻamaʻi le EDS76 antigen ua muaʻi ufiufi i luga ole enzyme micro-well strips.Whentesting, adddilutedserumsample, after incubation,pe afai e iai EDS76virusspecificantibody, o le a tuʻufaʻatasia ma le antigen pre-coated, lafoai le antibody e le tuʻufaʻatasia ma isi vaega i le fufuluina;ona fa'aopoopo lea o le enzyme fa'ailoga anti-EDS76 virus monoclonal antibody, ona tu'ufa'atasia lea o le antibody monoclonal ma le antigen ua fa'apipi'i muamua;tia'i le fa'atasi ole enzyme fa'atasi ma le fufulu;Fa'aopoopo le TMB substrate i micro-wells, o le faailo lanumoana e le Enzyme catalysis o loʻo i totonu o le faʻafeagai o mea o loʻo i totonu o le antibody i totonu o faʻataʻitaʻiga, faʻaaoga le ELISA reader i le 450nm wavelenth e fua ai le absorbance A tau i vaieli tali pe a uma ona faʻaopopo le fofo taofi.
e taofi le tali.
Mataupu
| Reagent | Volume 96 Su'ega/192Su'ega | ||
| 1 |
| 1ea/2ea | |
| 2 |
| 2.0ml | |
| 3 |
| 1.6ml | |
| 4 |
| 100ml | |
| 5 |
| 100ml | |
| 6 |
| 11/22ml | |
| 7 |
| 11/22ml | |
| 8 |
| 15ml | |
| 9 |
| 2ea/4ea | |
| 10 | serum dilution microplate | 1ea/2ea | |
| 11 | Faatonuga | 1 pcs |
